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OVERVIEW

The Perkins Monoclonal Antibody Facility produces pure, high quality antibodies needed for medical or research purposes to researchers and commercial clients. Researchers and biotechnology companies have access to the custom-made antibodies necessary to develop new products such as diagnostic tools. Monoclonal antibodies have been used to successfully treat breast cancer, lymphoma, rheumatoid arthritis, asthma, leukaemia and in transplants to minimise the chances of organ rejection.

Our services range from production of antisera to manufacture of gram amounts of pure monoclonal antibodies. Our specialty is in the production of custom monoclonal antibodies made with our high efficiency proprietary technology. We are one of the only suppliers with a strong track record in producing MAb to novel antigens that are particularly challenging.

The facility is managed by Kathleen Davern. Kathy has many years of experience in all aspects of monoclonal antibody production and has built on these strengths to expand both national and international capabilities.

The facility has an extremely satisfied Researchers, clinicians and commercial groups nationally and internationally have expressed their satisfaction with the value and efficiency of our services.

  • High efficiency monoclonal antibody production using the “Southern Cross”
  • Methods
  • Immunize various strains with GEX-biotin antigen
  • GEX (protein)-Biotin (small hapten)
  • Measure serum antibodies
  • Perform fusion
  • Compare:
    • antibody responses
    • Efficiency of MAb production

 

STRAIN No of Fusions Antibody Titre GEX Antibody Titre Biotin MAb +ve per fusion GEX MAb +ve  per fusion Biotin
C57BL/6 14 3200 3200 16 2
SC 073 5 120,000 50,000 65 25
SC 018 4 40,000 25,000 54 9
SC 104 4 7500 7500 15 13
SC 261 4 16,000 15,000 36 7
SC 424 7 2000 200 30 19
SC 038 3 600 900 11 4
SC 381 4 25,000 25,000 33 11
SC 027 3 75,000 40,000 55 22

We use “Southern Cross” strain SC 073 routinely for superior monoclonal antibody production.

SERVICES

The Facility provides a wide range of antibody-related products and services, including:

  • High efficiency custom production of monoclonal antibodies (MAb) using “Southern Cross”
  • Archive storage of cell lines
  • Polyclonal antibody production
  • Peptide synthesis and conjugation
  • In vitro production of MAbs in Bioreactors
  • Purification, conjugation and fragmentation of MAbs
  • Immunological assays

CAPABILITIES

Absolute client confidentiality
Large repository of valuable hybridoma lines and antibodies
University of Western Australia ethics approval
Production of MAb at Good Laboratory Practice level
Production of up to 1000 mg per run
State-of-the-art equipment for MAb production
Ability and support to expand the provision of products and services

Get in touch

Kathleen Davern
Monoclonal Antibody Facility
T. +61 8 6151 0739
E. [email protected]